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. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: Thromb Res. 2008 Jan 30;122(3):411–417. doi: 10.1016/j.thromres.2007.12.005

Figure 2.

Figure 2

Gla-peptide inhibition of BMP-2 induced transformation of pro-myoblast C2C12 cells into osteoblasts. Cells were plated in 96 well containing plates and cultured in CDMEM as described in MATERIALS AND METHODS for 48 hours in the presence of 5 nM of BMP-2 (open bar), 5 nM of BMP-2 + 0.5 mM Glu-peptide (black bar), 5 nM of BMP-2 + 0.5 mM Gla-peptide (cross hatched bar), 5 nM BMP-2 + 750 µM Gla-peptide (diagonal lines bar) and 5 nM BMP-2 + 43 nM of noggin respectively. ALP activities in the various wells measured as units (see MATERIALS AND METHODS) are shown for the various 48 hours cultured cells. Standard deviations are indicated as lines on the top of each bar in the graph. No ALP activity could be measured in pro-myoblasts and a P-value could not be given for the transformed cells.