Table 1.
Cell counts for immunohistochemical quantification are indicated. (A) The average number of counted orexin-positive cells, c-Fos positive cells, and the percentage of TH-positive cells double-labeled with c-Fos in the LH. (B) The average number of counted TH-positive cells, c-Fos positive cells, and the percentage of TH-positive cells double-labeled with c-Fos in the VTA and LC and the average number of c-Fos positive cells in the Acb. Values in parenthesis represent the standard error of the mean (SEM). Abbreviations used: LH, lateral hypothalamus; PFA, perifornical area; LLH, lateral region of the lateral hypothalamus; DMH, dorsal medial hypothalamus; Orx, orexin; Fos, c-Fos; TH, tyrosine hydroxylase; VTA, ventral tegmental area; LC, locus coeruleus; Acb, nucleus accumbens; AcbC, nucleus accumbens core; AcbSh, nucleus accumbens shell.
A. Orexin and c-Fos double-labeling in the LH
B TH and c-Fos double-labeling in the VTA and LC; c-Fos single-labeling in the NAc
| PFA | LLH | DMH | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Treatment | Fos+ | Orx+ | Percentage+ | Fos+ | Orx+ | Percentage+ | Fos+ | Orx+ | Percentage+ |
| Sal-Sal | 75.8(20.3) | 122.5(19.7) | 52.0(7.7) | 135.5(60.2) | 231.0(82.7) | 48.3(5.8) | 108.0(36.4) | 164.5(46.2) | 56.1(4.5) |
| SB-Sal | 86.3(18.7) | 148.0(18.9) | 56.9(7.3) | 117.0(23.5) | 296.0(45.8) | 39.5(6.4) | 158.0(14.8) | 230.6(16.2) | 68.7(5.1) |
| Sal-Nal | 95.0(11.6) | 132.3(15.7) | 71.7(2.0) | 164.3(21.9) | 277.2(34.6) | 59.2(2.1) | 132.3(11.8) | 163.6(15.5) | 81.1(1.1) |
| SB-Nal | 77.5(22.2) | 111.7(29.8) | 70.6(6.0) | 114.2(31.6) | 209.3(46.7) | 53.6(3.6) | 108.5(9.30) | 145.2(17.3) | 76.6(6.6) |
| VTA | LC | NAc Core | NAc Shell | |||||
|---|---|---|---|---|---|---|---|---|
| Treatment | Fos+ | TH+ | Percentage+ | Fos+ | TH+ | Percentage+ | Fos+ | Fos+ |
| Sal-Sal | 184.0(86.1) | 686.7(76.7) | 28.9(13.9) | 294.0(42.4) | 357.0(46.9) | 81.7(3.3) | 874.8(136.4) | 840.6(69.5) |
| SB-Sal | 105.0(32.5) | 464.0(196.2) | 33.7(14.2) | 297.0(30.7) | 369.0(8.5) | 80.2(6.6) | 934.6(52.6) | 924.2(59.9) |
| Sal-Nal | 260.0(118.9) | 771.3(58.1) | 31.9(12.3) | 284.7(27.4) | 353.2(30.0) | 80.4(2.3) | 1530.8(193.5) | 2321.8(170.0) |
| SB-Nal | 147.0(81.6) | 598.0(129.5) | 22.69(11.7) | 192.0(32.4) | 277.2(56.2) | 74.5(2.5) | 1677.8(139.6) | 1675.3(93.8) |