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. Author manuscript; available in PMC: 2008 Sep 4.
Published in final edited form as: J Am Chem Soc. 2006 Feb 1;128(4):1204–1213. doi: 10.1021/ja055313e

Table 2.

Thermodynamic parameters obtained from the thermal and chemical denaturant transition profilesa of WT RNase A and its Y92G/A/L mutants.

Proteins Tm (°C) EquationO (15 °C)b (kcal/mol) Enzymatic Activity (% of WT)
WT RNase A 61.9 ± 0.7 8.7 ± 0.6 100
Y92L 54.0 ± 0.2 7.2 ± 0.3 68
Y92A 55.8 ± 0.4 8.1 ± 0.4 56
Y92G 54.8 ± 0.6 7.3 ± 0.4 61
a

Data were obtained from UV absorbance measurements at 287 nm and at pH 8.

b

Standard free energy of unfolding.