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. Author manuscript; available in PMC: 2008 Sep 4.
Published in final edited form as: J Am Chem Soc. 2006 Feb 1;128(4):1204–1213. doi: 10.1021/ja055313e

Table 3.

Rate-constants for the reduction of disulfide bonds in WT and mutant RNase’s and in ONC (pH 8, 15 °C).

Protein Disulfide Bond Reduction Rate Constant (min−1M−1) Equation(kcal mol−1)
ONC (30–75) a2.3 ± 0.2 2.6 ± 0.1
WT RNase A (40–95) b(2.1 ± 0.5) × 10−3 6.6 ± 0.1
WT RNase A (65–72) b(2.3 ± 0.1) × 10−3 6.5 ± 0.1
Y92G RNase A (40–95) 1.44 ± 0.07 2.8 ± 0.1
Y92A RNase A (40–95) 0.133 ± 0.003 4.2 ± 0.1
Y92L RNase A (40–95) 0.091 ± 0.002 4.4 ± 0.1
a

From Narayan, M. et al. (20).

b

From Li, Y. L. et al. (17).