Fig. 4.

Validation of the GeneChip signals by real-time RT-PCR (A) and effect of Mn deficiency on the formation of root hairs in the cpc mutant (B, C). Gene expression analysis was performed with four (five for IRT1) RNA preparations from independently grown plants. Black bars indicate means of real-time PCR data, grey bars represent means of normalized GeneChip signals. Error bars shows standard deviation of the means. cpc mutants were grown in either media containing 14 μM Mn or in media deprived of Mn. Plants were analysed 6 d after sowing.