Table 2.
Expression profiles of Cu-responsive genes under excess Cu treatment conditions (10, 45, and 130 μM of CuCl2)
Probe ID | Full length cDNA | Locus_ID | Description |
F (experiment/control) |
||
Cu-exposure (μM) |
||||||
10 | 45 | 130 | ||||
Defence (up-regulated) | ||||||
A_71_P105870 | AK060724 | LOC_Os02g41630 | Phenylalanine ammonia-lyase | 1.02 | 1.70 | 2.01 |
A_71_P105867 | AK068993 | LOC_Os02g41680 | Phenylalanine ammonia-lyase | 1.01 | 1.42 | 5.01 |
A_71_P105871 | AK102817 | LOC_Os02g41630 | Phenylalanine ammonia-lyase | 1.19 | 1.82 | 2.26 |
A_71_P113211 | AK067801 | LOC_Os04g43800 | Phenylalanine ammonia-lyase | 1.34 | 1.78 | 4.61 |
A_71_P126860 | AK099443 | LOC_Os11g02440 | Chalcone-flavonone isomerase | 1.38 | 1.89 | 2.19 |
A_71_P104485 | AK070746 | LOC_Os02g08420 | Dihydroflavonol-4-reductase | 1.07 | 1.32 | 2.23 |
A_71_P119630 | AK065515 | LOC_Os08g38910 | Caffeoyl-CoA O-methyltransferase 2 | 1.19 | 2.12 | 3.18 |
A_71_P115157 | AK104994 | LOC_Os05g25640 | Trans-cinnamate 4-mono-oxygenase | 1.21 | 1.42 | 2.43 |
A_71_P123533 | AK069308 | LOC_Os10g02880 | O-methyltransferase ZRP4 | 1.17 | 1.19 | 4.27 |
A_71_P122641 | AK072740 | LOC_Os09g17560 | O-methyltransferase ZRP4 | 1.03 | 1.59 | 21.92 |
A_71_P111602 | AK065090 | LOC_Os04g59190 | Peroxidase 2 precursor | 1.38 | 1.79 | 7.16 |
A_71_P113417 | AK106200 | LOC_Os05g04500 | Peroxidase 63 precursor | 1.62 | 2.97 | 8.13 |
A_71_P117837 | AK072862 | LOC_Os07g47990 | Peroxidase 2 precursor | 1.34 | 1.37 | 3.50 |
A_71_P103756 | AK099241 | LOC_Os01g22370 | Peroxidase 1 precursor | 1.22 | 1.48 | 4.33 |
A_71_P120304 | AK069503 | LOC_Os08g02110 | Peroxidase 47 precursor | 1.20 | 1.31 | 3.30 |
A_71_P117839 | AK073202 | LOC_Os07g48020 | Peroxidase 2 precursor | 1.18 | 1.69 | 9.18 |
A_71_P103305 | AK107822 | LOC_Os01g72170 | Glutathione S-transferase | 1.21 | 1.24 | 2.07 |
A_71_P125246 | AK062653 | LOC_Os11g47809 | Metallothionein-like protein 1 | 1.37 | 1.48 | 4.06 |
Defence (down-regulated) | ||||||
A_71_P103051 | AK103129 | LOC_Os01g53330 | Anthocyanidin 5,3-O-glucosyltransferase | 0.80 | 0.58 | 0.29 |
A_71_P119739 | AK067868 | LOC_Os08g07880 | Phosphopantothenate-cysteine ligase | 0.61 | 0.48 | 0.43 |
A_71_P103162 | AK062796 | LOC_Os01g74300 | Metallothionein-like protein type 2 | 0.92 | 0.82 | 0.16 |
Response to stress (up-regulated) | ||||||
A_71_P112980 | AK100788 | LOC_Os04g34600 | ABA/WDS induced protein | 1.47 | 1.88 | 2.17 |
A_71_P115472 | AK107775 | LOC_Os06g07030 | Dehydration responsive element binding protein | 1.17 | 1.64 | 4.35 |
A_71_P126985 | AK062422 | LOC_Os09g35010 | Dehydration-responsive element-binding protein 1B | 1.24 | 1.86 | 2.27 |
A_71_P118699 | AK106022 | LOC_Os07g44250 | Disease resistance response protein 206 | 1.08 | 1.40 | 3.35 |
A_71_P111503 | AK071013 | LOC_Os04g41680 | Endochitinase A precursor | 1.09 | 1.14 | 2.45 |
A_71_P114512 | AK060312 | LOC_Os05g42230 | ER6 protein | 1.03 | 1.16 | 2.37 |
A_71_P124122 | AK065000 | LOC_Os10g22520 | Glucan 1,3-β-glucosidase precursor | 1.14 | 1.40 | 4.57 |
A_71_P121735 | AK061896 | LOC_Os09g30418 | Heat shock protein 81-3 | 1.37 | 1.78 | 2.39 |
A_71_P126129 | AK066682 | LOC_Os12g14440 | Jasmonate-induced protein | 1.63 | 1.99 | 11.92 |
A_71_P103425 | AK062520 | LOC_Os01g24710 | Salt stress-induced protein | 1.16 | 1.28 | 6.20 |
A_71_P114369 | AK070138 | LOC_Os05g28740 | Universal stress protein | 1.54 | 1.55 | 2.54 |
A_71_P114262 | AK065866 | LOC_Os05g15770 | Xylanase inhibitor protein 2 precursor | 2.01 | 2.09 | 4.34 |
A_71_P114261 | AK062114 | LOC_Os05g15770 | Xylanase inhibitor protein 2 precursor | 1.66 | 2.01 | 4.20 |
Response to stress (down-regulated) | ||||||
A_71_P117292 | AK099477 | LOC_Os06g47800 | Disease resistance protein RGA3 | 0.77 | 0.61 | 0.27 |
A_71_P118794 | AK065027 | LOC_Os07g01630 | Disease resistance response protein 206 | 0.95 | 0.77 | 0.49 |
A_71_P122593 | AK060664 | LOC_Os09g37600 | Erwinia-induced protein 1 | 0.94 | 0.76 | 0.43 |
Photosynthesis (up-regulated) | ||||||
A_71_P114297 | AK100910 | LOC_Os05g50380 | Glucose-1-phosphate adenylyltransferase large subunit, chloroplast precursor | 1.39 | 1.47 | 3.93 |
A_71_P116411 | AK101836 | LOC_Os06g49110 | Δ-Aminolevulinic acid dehydratase, chloroplast precursor | 1.39 | 1.73 | 2.03 |
Photosynthesis (down-regulated) | ||||||
A_71_P105099 | AK062994 | LOC_Os02g51470 | ATP synthase delta chain, chloroplast precursor | 0.96 | 0.87 | 0.45 |
A_71_P115841 | AK060904 | LOC_Os06g21590 | Chlorophyll a-b binding protein 6A, chloroplast precursor | 0.81 | 0.71 | 0.48 |
A_71_P125058 | AK061295 | LOC_Os11g13890 | Chlorophyll a-b binding protein M9, chloroplast precursor | 0.70 | 0.63 | 0.37 |
A_71_P118301 | AK109399 | LOC_Os07g37550 | Chlorophyll a-b binding protein of LHCII type III, chloroplast precursor | 0.69 | 0.58 | 0.40 |
A_71_P121584 | AK109203 | LOC_Os09g32620 | Chloroplastic quinone-oxidoreductase | 0.76 | 0.73 | 0.48 |
A_71_P101901 | AK066307 | LOC_Os12g10604 | Cytochrome b/b6/petB family protein | 0.61 | 0.54 | 0.32 |
A_71_P126393 | AK059037 | LOC_Os12g08770 | Photosystem I reaction centre subunit N, chloroplast precursor | 0.73 | 0.67 | 0.40 |
A_71_P114565 | AK066345 | LOC_Os05g43310 | Photosystem II reaction centre W protein, chloroplast precursor | 0.76 | 0.76 | 0.40 |
A_71_P108389 | AK058858 | LOC_Os03g55720 | Plastoquinol-plastocyanin reductase | 0.94 | 0.73 | 0.36 |
A_71_P117917 | AK069170 | LOC_Os07g36080 | Oxygen-evolving enhancer protein 3-1, chloroplast precursor | 0.93 | 0.80 | 0.30 |
A_71_P117916 | AK058793 | LOC_Os07g36080 | Oxygen-evolving enhancer protein 3-1, chloroplast precursor | 0.70 | 0.61 | 0.29 |
A_71_P120166 | AK058551 | LOC_Os08g25734 | Glucose-1-phosphate adenylyltransferase small subunit, chloroplast precursor | 0.86 | 0.84 | 0.47 |
A_71_P124217 | AK110705 | LOC_Os06g39730 | Ribulose bisphosphate carboxylase large chain, catalytic domain containing protein | 0.76 | 0.73 | 0.50 |
Transport (up-regulated) | ||||||
A_71_P105105 | AK108711 | LOC_Os02g34580 | Ammonium transporter 2 | 1.06 | 1.35 | 2.47 |
A_71_P119764 | AK065217 | LOC_Os08g03350 | LHT1 | 1.19 | 1.36 | 2.46 |
A_71_P117869 | AK105311 | LOC_Os07g33780 | PDR-like ABC transporter | 1.09 | 1.17 | 2.61 |
A_71_P127448 | AK108393 | LOC_Os05g27010 | Peptide transporter PTR2 | 1.17 | 1.33 | 2.22 |
A_71_P103242 | AK063835 | LOC_Os01g45640 | Tat pathway signal sequence family protein | 1.02 | 1.04 | 7.43 |
A_71_P100920 | AK103784 | LOC_Os01g31980 | Transparent testa 12 protein | 1.10 | 1.43 | 2.56 |
Transport (down-regulated) | ||||||
A_71_P106018 | AK100650 | LOC_Os02g44980 | Amino acid transport protein | 0.99 | 0.76 | 0.45 |
A_71_P116013 | AK107472 | LOC_Os06g12320 | Amino acid/polyamine transporter II | 0.80 | 0.68 | 0.22 |
A_71_P115705 | AK072617 | LOC_Os06g03700 | Oligopeptide transporter 9 | 0.87 | 0.84 | 0.24 |
A_71_P104541 | AK065840 | LOC_Os02g46460 | Peptide transporter PTR2 | 0.74 | 0.57 | 0.29 |
A_71_P122896 | AK066937 | LOC_Os10g42900 | Peptide transporter PTR2 | 0.77 | 0.73 | 0.49 |
A_71_P114702 | AK070558 | LOC_Os05g34010 | Peptide transporter PTR2 | 0.90 | 0.77 | 0.48 |
A_71_P102553 | AK066793 | LOC_Os01g50616 | Phosphatidylinositol transporter/ transporter | 0.75 | 0.68 | 0.44 |
A_71_P119359 | AK066067 | LOC_Os07g46780 | Tyrosine-specific transport protein | 0.67 | 0.64 | 0.41 |
A_71_P123937 | AK111957 | LOC_Os10g38910 | ABC-type Co2+ transport system, permease component | 0.91 | 0.73 | 0.44 |
A_71_P115940 | AK105826 | LOC_Os06g30730 | ATPase, coupled to transmembrane movement of substances | 0.99 | 0.75 | 0.48 |
A_71_P100064 | AK065048 | LOC_Os01g17214 | Carbohydrate transporter/sugar transporter/transporter | 0.86 | 0.63 | 0.22 |
A_71_P123327 | AK071193 | LOC_Os10g35140 | Permeases of the drug/metabolite transporter | 0.82 | 0.68 | 0.50 |
A_71_P104342 | AK071338 | LOC_Os02g56510 | Phosphate transporter 1 | 0.59 | 0.54 | 0.42 |
A_71_P117558 | AK067110 | LOC_Os06g29790 | Phosphate transporter 1 | 0.55 | 0.48 | 0.27 |
A_71_P112325 | AK070018 | LOC_Os04g38026 | Sugar transport protein 5 | 0.74 | 0.63 | 0.37 |
A_71_P108667 | AK067353 | LOC_Os03g09930 | Sulphate transporter 2.1 | 0.53 | 0.39 | 0.10 |
A_71_P112060 | AK072809 | LOC_Os04g55800 | Sulphate transporter 3.3 | 0.90 | 0.84 | 0.49 |
A_71_P116372 | AK063490 | LOC_Os06g36450 | Transporter like protein | 0.92 | 0.66 | 0.39 |
Values are means of fold-change (F) calculated from triplicate data of different leaves. The descriptions of each gene were annotated according to the TIGR database (http://www.tigr.org/tdb/e2k1/osa1/), and were classified into rough functional categories based on the Gene Ontology Classification database (http://www.geneontology.org/).