Fig. 3.

Differential expression of peroxidase and CCoAOMT isogenes in the skin compared to tuber flesh. Primers corresponding to peroxidase and CCoAOMT isozymes were designed based on specific peptide sequences obtained from the MS analysis and were used in semi-quantitative RT-PCR with RNA samples of skin (white bars) and tuber flesh (grey bars). The expression levels were monitored relative to 18S rRNA levels in each sample. NAC protein (TA25576, spot 46) was used as reference gene for equal expression in skin and flesh tissues. Signal intensities were quantified by Image Gauge software.