Table 2.
List of skin proteins that accumulate differentially in potato tuber skin compared to tuber flesh
Spot no. | Functional categories | Potato TAa/EST | Match peptidesb | Cover (%)c | MW(kDa)/pI |
|
Experimental | Theoretical | |||||
Cell proliferation | ||||||
1 | Actin (ACT) | TA24741 | 10 | 21 | 43/4.8 | 41.8/5.31 |
2d | P23 tumor protein-like (P23/TCTP) | TA24105 | 2 | 10 | 17.84/4.84 | 18.85/4.58 |
3d | Proteasome α-7 subunit | TA26206 | 6 | 20 | 27.6/6.3 | 27.1/7 |
4 | Proteasome β-2A subunit | TA32826 | 8 | 30 | 21.1/6.48 | 22.1/6.2 |
5 | Translation initiation factor 5A-3, eukaryotic (eIF-5A3) | TA23158 | 6 | 30 | 16.25/6.25 | 17.57/5.78 |
6 | Tubulin α-chain | TA24049 | 11 | 32 | 49/5.2 | 49.75/4.93 |
Signal transduction—cell wall | ||||||
7 | Remorin (REM) | DN921712 | 4 | 16 | 30.4/6.14 | 21.8/6.14 |
General metabolism | ||||||
8d | UDP-glucose:protein transglucosylase (UPTG2) | TA24497 | 9 | 21 | 36.9/5.8 | 41.15/5.61 |
8d | Disulphide-isomerase protein (PDI) | TA25269 | 5 | 17 | 36.9/5.8 | 39.52/5.62 |
9d | Triosephosphate isomerase, (TPI) cytosolic isoform | TA25726 | 3 | 16 | 24.4/5.8 | 21.6/5.7 |
Oxidative respiratory chain | ||||||
3d | APFI (hypothetical protein F8G22.2) | TA26786 | 4 | 12 | 27.6/6.3 | 29.5/6.8 |
2d | NADH-ubiquinone oxidoreductase 18 kDa subunit, mitochondrial precursor | TA29219 | 2 | 6 | 17.84/4.84 | 19.76/4.94 |
10 | NADH:FMN oxidoreductase-like protein | TA30877 | 5 | 22 | 21.1/6.38 | 21.5/6.2 |
One-carbon (C1) metabolism | ||||||
11 | Glutamate-ammonia ligase (GS1) | TA25277 | 5 | 21 | 38/4.5–4.8 | 38.7/5.6 |
12 | Serine hydroxymethyltransferase 4 (SHMT4) | TA24707 | 10 | 28 | 53.6/6.4 | 51.7/6.8e |
13, 14 | Methionine synthase (MS) | TA24454 | 11–16 | 19–30 | 78.5/6.46–6.57 | 84.6/5.93 |
Abiotic and biotic stress | ||||||
15 | Plasma-membrane polypeptide (DREPP) | TA26238 | 9 | 25 | 26/5.2 | 21.9/5.02 |
Oxidative stress | ||||||
16 | Ascorbate peroxidase 1 (APX1), cytosolic | TA24251 | 12 | 35 | 25.4/5.93 | 27.47/5.56 |
17 | Catalase isozyme 2 (CAT2) | TA23780 | 17 | 35 | 53.6/6.4 | 56.5/6.56 |
18 | Catechol oxidase B, chloroplast precursor | TA25457 | 6 | 10 | 58.2/5.9 | 66.4/6.3 |
19f | Polyphenol oxidase (PPO) | TA24911 | 7 | 11 | 56/6 | 66.7/6.8 |
Plant defence | ||||||
20d | Cysteine protease 1 (CYP1) | CK256743 | 3 | 8 | 38.5/4.86 | 32/5.8 |
21 | Elicitor-inducible protein EIG-J7 | TA32425 | 3 | 12 | 16.1/6.57 | 20/6.88 |
22f | Elicitor-inducible protein EIG-J7 | TA39811 | 4 | 15 | 15/4.86 | 20.3/6.08e |
23 | Endochitinase 2 precursor | TA24214 | 4 | 18 | 28/6.1 | 34.3/5.93 |
24 | Endochitinase 2 precursor | CN213814 | 2 | 11 | 27.6/6.3 | 28.6/7.48 |
20d,25–28 | Patatin | TA23294 | 9 | 10–27 | 39.6/5.26 | 43.4/5.11 |
29 | Patatin putative homolog | TA35081 | 5 | 14 | 43/6 | 43.5/6.5 |
30,31 | Patatin protein 07 | TA23344 | 11 | 26 | 36.7–38.5/5.36–5.6 | 42.6/5.2 |
32 | Pathogenesis-related protein 10 (PR-10) | CN213132 | 10 | 38 | 16.5/5.45 | 17.4/5.3 |
33f | Pathogenesis-related protein 10 (PR-10) | TA22962 | 3 | 13 | 16.9/5.5 | 17.4/5.3 |
34 | 2-Oxoglutarate-dependent dioxygenase (SPP2) | TA24517 | 3 | 9 | 36.7/5.6 | 37.9/5.54 |
Suberization/lignification | ||||||
35 | ACP-17 kDa β-hydroxyacyl-acyl carrier protein | TA29588 | 6 | 17 | 17.63/6.15 | 24.6/9.03 |
9d | Caffeoyl-CoA O-methyltransferase-5 (CCoAOMT-5) | TA33337 | 6 | 26 | 24.4/5.8 | 26.3/5.51 |
36 | Caffeoyl-CoA O-methyltransferase-6 (CCoAOMT-6) | TA30542 | 7 | 29 | 26.4/5.48 | 27.98/5.4 |
37 | Caffeoyl-CoA O-methyltransferase-3 (CCoAOMT-3) | TA30485 | 4 | 14 | 26/4.8 | 25.95/5.4e |
38,39 | Peroxidase (POD 18) | TA25873 | 10–11 | 27–28 | 32.8/ 6.4–6.7 | 36.3/6.32 |
40 | Peroxidase PER9-6 secretory (POD 20) | TA23216 | 12 | 22 | 43/6.8 | 39.7/7.6 |
41 | Peroxidase 136, class III, precursor (POD 9) | TA39454 | 10 | 34 | 32.8/5.35 | 35/5.2 |
42 | Peroxidase putative (POD 5) | CV504265 | 3 | 12 | 39.6/4.85 | 35.85/9.2e |
43-45 | Peroxidase, suberization-associated anionic peroxidase (POP_A) | TA25019 | 5 | 15 | 53.4–39.6/4.15–4.2 | 36.3/6.32 |
Reference protein | ||||||
46 | Nascent polypeptide-associated complex NAC; UBA-like | TA25576 | 4 | 20 | 24.9/4.42 | 21.7/4.38 |
Potato Transcript Assemblies (http://plantta.tigr.org/) and EST identifiers.
Number of peptides identified.
Sequence coverage in between (%).
Spot that contains more than one protein.
Calculated using the most similar protein.
Additional isozymes were found in the skin Table S2.