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. 2008 Jul 31;59(12):3475–3484. doi: 10.1093/jxb/ern197

Fig. 1.

Fig. 1.

BjCHI1 inhibits growth of phytopathogens Colletotrichum truncatum, C. acutatum, Botrytis cinerea, and Ascochyta rabiei. (A, B) Photographs of the bottom of Petri plates showing the effect of BjCHI1 and its derivative with one chitin-binding domain on the growth of C. truncatum (A) and C. acutatum (B). FPLC-purified proteins were added in wells when the hyphal front was 0.5 cm from the wells. Well 1, control with 10 mM sodium phosphate buffer (pH 7.2); well 2, 5 μg BjCHI1; well 3, 20 μg BjCHI1; well 4, 5 μg BjCHI1 derivative, and well 5, 20 μg BjCHI1 derivative. The photograph was taken 72 h after addition of protein. (C, D) Photographs of the top of the plates showing the effect of BjCHI1 on the growth of C. acutatum (C) and B. cinerea (D). Well 1, control with 10 mM sodium phosphate buffer (pH 7.2); well 2, 10 μg BjCHI1; well 3, 20 μg BjCHI1; well 4, 30 μg BjCHI1; well 5, 40 μg BjCHI1 and well 6, 50 μg BjCHI1. (E) Photograph of the top of the plate showing the effect of BjCHI1 on the growth of A. rabiei. Well 1, control with 10 mM sodium phosphate buffer (pH 7.2); well 2, 4 μg BjCHI1; well 3, 8 μg BjCHI1; well 4, 12 μg BjCHI1; well 5, 16 μg BjCHI1, and well 6, 20 μg BjCHI1.