Table 1.
Triple quadrupole tune settings for the specific standard peptides used for absolute quantification
| TC code | Name | Peptide sequence | Standard peptide |
Native peptide |
||||
| Precursor ion (m/z) | Product ions (m/z) | Collision energy | Precursor ion (m/z) | Productions (m/z) | Collision energy | |||
| TC100391 | AS | STYAWGLEAR | 580.7 | 738.3 | 19 | 577.2 | 731.3 | 19 |
| TC100393 | AS | STFAWGLEAR | 572.8 | 552.2 | 18 | 569.3 | 545.2 | 18 |
| TC106729 | GS1a | ITEIAGVVVSFDPK | 741.4 | 243.9 | 36 | 737.9 | 243.9 | 36 |
| TC106808 | GS1b | HETADINTFLWGVANR | 617.6 | 702.3 | 19 | 615.3 | 702.3 | 19 |
| TC106913 | GS2 | IHIEAYGEGNER | 465.5 | 475.1 | 14 | 463.2 | 475.1 | 14 |
| TC94780 | GOGAT | EVLVDFDNLLPK | 704.9 | 1166 | 17 | 701.4 | 1159 | 17 |
| TC94631 | AAT2 | ATAELLLGADNPAIK | 752.4 | 785.4 | 22 | 748.9 | 785.4 | 22 |
| TC106918 | AAT1 | TEEGKPLVLDVVR | 487.9 | 917.6 | 19 | 485.6 | 910.6 | 19 |
| TC94623 | AAT | IPSGHGYDDFEVVR | 534.2 | 744.3 | 18 | 530.9 | 739.3 | 18 |
| TC94704 | GDH | GLDIPSLLK | 481.8 | 564.3 | 15 | 478.3 | 557.3 | 15 |
| TC100410 | SuSy1 | VHSLKER | 438.2 | 639.3 | 18 | 434.7 | 632.3 | 18 |
| TC100410 | SuSy1 | ALESEMLSR | 521.7 | 851.4 | 18 | 518.2 | 851.4 | 18 |
| TC95820 | SuSy2 | ALENEMLAR | 527.2 | 862.4 | 18 | 523.7 | 862.4 | 18 |
| TC94447 | SuSy3 | ALENEMLR | 491.7 | 791.3 | 17 | 488.2 | 791.3 | 17 |
| TC99016 | SuSy5 | ALEEELLQK | 540.2 | 888.5 | 18 | 536.7 | 888.5 | 18 |
Peptide sequences employed in this work, as well as their corresponding precursor masses and product ions are given. Stable isotope-labelled leucine residues are marked in bold.