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. 2008 Aug 6;59(12):3485–3499. doi: 10.1093/jxb/ern201

Fig. 5.

Fig. 5.

Characterization of the Atscp2-1 mutant. In (A) is the genomic structure of the AtSCP2 loci on chromosome 5 of the A. thaliana genome. Exons are indicated as bars. The location of the T-DNA insertion in Atscp2-1 is shown. (B) PCR analysis of the AtSCP2 loci in genomic DNA from A. thaliana wild-type and Atscp2-1. The absence of the AtSCP2 specific (arrow) PCR product indicates that the Atscp2-1 line is homozygous for the T-DNA insertion. (C) Analysis of the expression of AtSCP2 mRNA in wild-type Col-0 and the Atscp2-1 line. The absence of a PCR product from RT-PCR analysis of AtSCP2 cDNA shows that AtSCP2 is not expressed in the Atscp2-1 line. Information regarding primers is provided in the Materials and methods.