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. Author manuscript; available in PMC: 2008 Sep 4.
Published in final edited form as: Anal Chem. 2007 Jan 1;79(1):161–167. doi: 10.1021/ac061270o

Figure 1.

Figure 1

Schematic picture of the experimental setup. (A) Cultured cells were mounted in a chamber on the microscope stage. The tapered outlet end of the capillary was positioned close to a fluorescent cell with the help of a micromanipulator. The inlet end of the capillary was placed in a buffer-filled vial. The setup was connected to a high voltage power supply. (B) Single pulse of varying pulse durations (50 ms −300 ms) were applied at 500 V. This resulted in the release of fluorescent thiols from the cell interior.