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. Author manuscript; available in PMC: 2008 Sep 4.
Published in final edited form as: Anal Chem. 2007 Jan 1;79(1):161–167. doi: 10.1021/ac061270o

Figure 3.

Figure 3

High spatial resolution achieved with single cell electroporation. Plated cells were incubated with 2 μM Thioglo-1 in the intracellular buffer. A single pulse of 200 ms at a cell-capillary tip distance of 2.0 μm was applied at 500 V. Images were collected at a frequency of one frame per second. (A) Fluorescence micrograph of 2 fluorescent cells ∼ 10 μm from each other. The arrow shows the approximate position of the capillary. The images were taken before pulsation (0 s), and after pulsation (30 s, and 2 min after the start of the acquisition). (C) Normalized average fluorescence intensity for cells A and B (B) against time. All the data were corrected for bleaching.