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. 2008 Aug 12;9(1):60. doi: 10.1186/1465-9921-9-60

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Copyright © 2008 Sullivan et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Validation of the ELISA for measuring SLPI with proteinases. Recombinant human SLPI was mixed with varying concentrations of NE and assayed by ELISA to assess the recovery of SLPI in complex with the proteinase and in the presence of excess proteinase. The molar ratio of proteinase to SLPI is given along the x axis, and the percentage of SLPI recovered is shown on the y axis. The figure shows the results obtained with NE, CG, PPE and trypsin. The ELISA was affected by a large molar excess of proteinase, but not at the molar ratios relevant to this paper (< 10:1).