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. 2008 Jul 25;8:219. doi: 10.1186/1471-2148-8-219

Figure 4.

Figure 4

LampER is activated by E2 whereas amphiER is not activated by ER agonists. The pSG5 constructs containing either amphiER (A), human ERα or human ERβ (B), were tested in transfected Cos 7 cells for their ability to activate the co-transfected cognate ERE-luc reporter plasmid after E2 stimulation (10-6M). (C) GAL4-LBD constructs from several chordate ERs were tested in transfectec 293 cells for their ability to activate a (17m)5x-G-luc reporter plasmid in the presence of increasing doses of E2 (10-9M to 10-6M). (D) Mammalian two-hybrid SRC1 recruitment assay. The GAL4-amphiER-LBD chimera was used with the coactivator SRC1 fused to the strong activation domain VP16 to transfect 293 cells in the presence of increasing doses of E2 (10-9M to 10-6M).