Table 2.
Characteristics of used primers; LNA probes and amplicon sizes in reverse transcriptase-polymerase chain reaction reaction.
| Gene abbreviation | Amplicon size (basepairs) | Sense, antisense primers | LNA probe¥ |
| ACTB | 76 | 5'-attggcaatgagcggttc-3' 5'-ggatgccacaggactccat-3' |
#11 |
| ARF1 | 70 | 5'-gccactacttccagaacacaca-3' 5'-tcgttcacacgctctctgtc-3' |
#56 |
| CANX | 108 | 5'-aacaccagaactcaacctgga-3' 5'-tgtcggaagatgaagtgcag-3' |
#55 |
| G6PD | 75 | 5'-ctggtggccatggagaag-3' 5'-gcatttcaacaccttgacctt-3' |
#22 |
| GAPDH | 78 | 5'-tccactggcgtcttcacc-3' 5'-ggcagagatgatgaccctttt-3' |
#45 |
| GNB2L1 | 72 | 5'-gctactaccccgcagttcc-3' 5'-cagtttccacatgatgatggtc-3' |
#55 |
| GPS1 | 66 | 5'-gcaaccagatccatgtcaagt-3' 5'-tgttggctggagtcagctc-3' |
#36 |
| PSMB2 | 72 | 5'-agagggcagtggaactcctt-3' 5'-aggttggcagattcaggatg-3' |
#50 |
| PSMD2 | 68 | 5'-gcctcacccagattgacaag-3' 5'-ggcaagaagagctcctgactta-3' |
#82 |
| RPL32 | 75 | 5'-gaagttcctggtccacaacg-3' 5'-gcgatctcggcacagtaag-3' |
#17 |
| INFG | 112 | 5'-ggcattttgaagaattggaaag-3' 5'-tttggatgctctggtcatctt-3' |
#21 |
| CCL2 | 93 | 5'-agtctctgccgcccttct-3' 5'-gtgactggggcattgattg-3' |
#40 |
¥Numbers of LNA probes according to the commercially available library http://www.universalprobelibrary.com