Figure 4.
Low-density lipoprotein receptor, a known substrate of PCSK9, is acetylated. (A) LDLr was immunoprecipitated from stably transfected CHO cells and analysed by immunoblotting with the indicated antibodies. (B) LDLr was purified by affinity chromatography (ProFound system) and incubated in the presence of HAT and [3H]acetyl-CoA for 1 h at 30°C (Costantini et al, 2007). LDLr was then immunoprecipitated and analysed on a scintillation liquid counter. The results are the average (n=4)±s.d.; *P<0.0005. Ac. Lys., acetylated lysine antibody; CHO, Chinese hamster ovary; HAT, histone acetyltransferase; LDLr, low-density lipoprotein receptor; PCSK9, proprotein convertase subtilisin kexin type 9.