Figure 4ad.

Disruption of the blood–testis barrier in fads2−/− males. Confocal images of cryosections of seminiferous tubuli of adult (2 months) wt and fads2−/− littermates. Double immunofluorescence labelling of TJ: (A, B) JAM-A (anti-rabbit IgG Alexa 488, green) and occludin (anti-goat IgG Cy3, red), (C, D) ZO-1 (green) and occludin, (E, F) claudin 11 (green) and occludin, stained with their respective antibodies using DAPI nuclear staining. (G, H) Anti-Cx43, (I, J) adherent junctions with anti-β-catenin antibodies (red) and TO-PRO-3 (blue) for nuclear staining. (K, L) G-actin stained with anti-G-actin antibodies (green) and F-actin with phalloidin (red). Dashed white lines mark the basal lamina. Magnification × 63. (M) Western blot analysis and densitometric quantification of TJ-specific JAM-A, GJ Cx43 and AJ β-catenin in the protein extracts of wt and fads2−/− testes. β-Tubulin was used as a loading marker. Arrows highlight the important changes.