Figure 2.
Bud32p kinase activity is inhibited by Kae1p. The phosphotransferase activities of Bud32p and the Bud32p/Kae1p complex on Grx4p and dephosphorylated casein were tested. PhosphoImager scan (upper panel) and Coomassie blue staining of SDS–PAGE gels (lower panel) of the reaction mixtures are shown.