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. 1998 Mar;9(3):653–670. doi: 10.1091/mbc.9.3.653

Figure 5.

Figure 5

Immunofluorescent localization of Kex2p and Och1p in arf mutant cells. Wild-type (SEY6210.5 pKE2018) and arf1Δ (6210.5 arf1Δ pKE2018) cells overexpressing Kex2p and arf1Δ cells expressing Och1-HA (6210.5 arf1Δ pOH URA3) were stained using affinity-purified antibody to Kex2p (A and C) or a monoclonal antibody to the HA epitope tag (E). (B, D, and F) Images captured using DIC optics correspond to the adjacent fluorescent image. Ring structures were apparent in most arf1Δ cells stained for Och1-HA, whereas only ∼10% of arf1Δ cells exhibited rings when stained for Kex2p. (G and H) An arf1–3 ts mutant (strain C156–1B, G–H) transformed with pOH was preincubated for 1 h at 37°C before fixation and immunofluorescence localization of Och1p.