Graphical representation of [35S]-labeled His×6-GMAP-210 binding scintillation counting assay for bis-Ni-NTA nanoparticles (solid bars) and commercial Ni-NTA agarose beads (open bars). Fractions collected and radioactivity quantified in counts per minute (CPM), DL = Direct Lysate, FT = Flow Through, W1 = Wash 1, W2 = Wash 2, W3 = Wash 3, E1 = Elution 1, E2 = Elution 2.