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Binding assay of His×6-rmES: (A) Commercial magnetic agarose beads (Qiagen): no protein was bound to the bead or eluted by imidazole. Most of the protein was washed away unbound in the FT. (B) Bis-Ni-NTA NPs: His×6-tag protein was bound and a majority was eluted from the nanoparticles by imidazole. (C) Graphical representation of densitometry quantification of SDS-PAGE gels.
