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. Author manuscript; available in PMC: 2008 Sep 5.
Published in final edited form as: Bioconjug Chem. 2007 Feb 21;18(2):333–341. doi: 10.1021/bc060195l

Figure 5.

Figure 5

Preference toward nonspecific interaction before his-tag—Ni-NTA interaction demonstrated through increasing ratios of 6×His-rmES protein (μg) to bis-Ni-NTA nanoparticle (mg) A: 10.2, B: 15.3, C: 61.3. (M = Marker, FT = Flow Through, W1 = Wash 1, W2= Wash 2, Wash 3 = W3, E1 = Elution 1, E2 = Elution 2, NP = protein bound nonspecifically to nanoparticle.)