Skip to main content
. Author manuscript; available in PMC: 2008 Sep 5.
Published in final edited form as: Biochemistry. 2007 Apr 3;46(16):4763–4774. doi: 10.1021/bi062208o

Figure 5.

Figure 5

Putative Ets sites located in the−515 to −805 bp region of the mouse TSPO promoter are strong positive elements regulating transcriptional activity. A. Luciferase reporter assays were performed in MA-10 and NIH/3T3 cells using constructs carrying 2 bp substitution mutations for Ets (pGL3-805mEts), AR/PR (pGL3-805mA/P), and both Ets and AR/PR (pGL3–805mE/A/P). B. Luciferase reported assays were performed in MA-10 and NIH/3T3 cells using constructs carrying identical 2 bp substitution mutations in the context of the upstream 805 bp fragment (pGL3-805mEts.2) and the upstream 585 bp fragment (pGL3-585mEts.2). A reporter construct carrying 2 bp substitution mutations on both Ets sites (pGL3-805mEts.1/2) was also examined. Results are expressed as a percentage of the activity of the WT promoter construct (pGL3-805). *p <0.05. The transcription initiation site is indicated by an arrow.