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. Author manuscript; available in PMC: 2008 Sep 5.
Published in final edited form as: Biochemistry. 2007 Apr 3;46(16):4763–4774. doi: 10.1021/bi062208o

Figure 6.

Figure 6

GABPα binds to its corresponding putative binding site in vitro. Electrophoretic mobility shift assay was performed using double-stranded, biotinylated oligonucleotide Ets. and nuclear extract from NIH/3T3, MA-10, or Y-1 cells. Competition experiments with unlabeled probe were performed using Ets.1 oligonucleotide (lanes 3, 11, 13, and 21), mutant oligonucleotides Ets-m1 and Ets-m2 (lanes 4 and 5), mutant oligonucleotides AR/PR-m1 and AR/PR-m2 (lanes 6 and 7), and an unrelated oligonucleotide (lane 8). Super-shift analysis was performed using antibodies specific for Ets1 Ets2, Elk1 and GABPα (lanes 15-18). An arrow indicates specific complex, and the arrow head indicates free, unbound probe.