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. Author manuscript; available in PMC: 2009 Jul 18.
Published in final edited form as: J Mol Biol. 2008 May 20;380(4):598–607. doi: 10.1016/j.jmb.2008.05.032

Figure 4. Contribution of MuB to Mu insertion within hot and cold targets in vitro.

Figure 4

A. Strand transfer reactions carried out in the presence of MuB included either ATP or ATPγS, and employed min-Mu plasmids as donor and linear DNA as target. MuB concentration was 160nM. Reactions in the absence of MuB employed precleaved oligonucleotide Mu R ends as donors. Insertions were analysed as in Fig. 3. B, C. Reactions using the yidP target with increasing MuB concentrations in the presence of ATP or ATPγS.