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Determination of the relative strength of two-hybrid interactions. β-Galactosidase activities (arbitrary units) were measured in total lysates of yeast containing an LacZ-reporter plasmid. The yeast cells contained plasmids encoding baits as indicated below the bars, and the empty prey vector pJG4–5 (A, gray bars), hRIL(164–330 (B, filled bars), or mRIL-FL (C, open bars). Error bars indicate SD (n = 5).
