Figure 1.

(A) Schematic representation of the wild-type and mutated human FGF-2 cDNAs used to stably transfect NIH 3T3 cells. The strategy used to produce these constructs is described in MATERIALS AND METHODS. (B) Amino acid sequence of the human FGF-2 N-terminal extension. Eight potential sites for arginine methylation exist (bold characters) according to the consensus sequence requirements reported by Najbauer et al. (1993). The underlined letters indicate the three arginines that were found to be methylated in the guinea pig brain HMW FGF-2. The arrows indicate the start sites for the translation of the 24-, 22.5-, 22-, and 18-kDa FGF-2 forms.