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Colony formation in soft agar of cells expressing different FGF-2 forms. Cells (25,000 or 50,000) were plated in agar (0.3%), and feeding was performed every 5 d as detailed in MATERIALS AND METHODS. After 25 d colonies were counted and expressed as percentage of the total number of cells plated. (A) Clones overexpressing HMW forms of FGF-2. (B) Clones overexpressing NLS-FGF-2 chimeras. Data represent the mean ± SD of representative experiments of three performed.
