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Hypo-osmotic conditions induce the tubulation and redistribution of GPP130 to the ER. COS-7 cells, grown to 50% confluence on 12-mm glass coverslips, were removed from normal medium and placed in 1 ml of hypotonic (210-mOsm) medium for 30 min (B) or 120 min (C) or hypotonic medium plus 0.1 M sucrose (D) for 30 min. Cells maintained in normal medium are shown in A. Cells were fixed and processed for immunofluorescence using a mAb against GPP130.
