Figure 6.

Hypo-osmotic conditions induce the redistribution of other Golgi residents to the ER. HeLa cells, grown to 50% confluence on 12-mm glass coverslips, were removed from normal medium and placed in 1 ml of hypotonic medium for 5 min (B and F), 30 min (C and G), or 120 min (D and H). The inset in B shows an enlargement of a portion of the cell shown in B. Cells maintained in normal medium are shown in A and E. Cells were fixed and processed for double-label immunofluorescence using a polyclonal antiserum against GM130 (A–D) and a mAb against GPP130 (E–H).