Figure 1.

Schematic representation of β1A, β1B, and cytoplasmic domain deletion mutants prepared for these studies. The amino acid sequences of β1A and β1B cytoplasmic domain are indicated. The β1COM mutant was truncated at threonine 782 and contains only the subdomain common to the different human β1 isoforms; the β1TR mutant was truncated at isoleucine residue 762 and lacks the entire cytoplasmic domain; the β1ΔCOM-A and β1ΔCOM-B lack the common subdomain, so that the distal A and B subdomains, respectively, are directly linked to the transmembrane segment. These mutants were prepared by oligonucleotide-mediated and recombinant PCR mutagenesis as described in MATERIALS AND METHODS.