Figure 11.

Association of talin and α-actinin with β1 cytoplasmic domain variants. CHO cells transfected with β1B, β1A, β1COM, and β1TR integrin variants were incubated in suspension with TS2/16 mAb to human β1, and cells were lysated in digitonin-containing buffer. Immunocomplexes were recovered from cell extracts with protein G-Sepharose beads and analyzed by Western blotting with talin antibody. The blot was stripped and reprobed with α-actinin antibody. Arrowheads point to the talin and α-actinin bands.