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. 1998 Apr;9(4):715–731. doi: 10.1091/mbc.9.4.715

Figure 4.

Figure 4

Effect of Mn++ on the conformation of β1B, β1A, and the four mutants as detected by 12G10 mAb. (A) GD25-β1B (left) and GD25-β1A (right) cells were analyzed by flow cytometry for surface expression of the 12G10 mAb epitope in the presence or absence of 1 mM Mn++. As a control, cells were also stained with mAb 13, which recognizes an Mn++-insensitive β1 epitope. GD25 cells were used as a negative control. (B and C) The histograms show the level of expression of the 12G10 mAb epitope in β1-transfected GD25 (B) and CHO (C) cells in the absence (open bars) or presence (striped bars) of 1 mM Mn++. Mean fluorescence intensities are expressed ± SE as percentage of mAb 13 values and represent the mean of five experiments.