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Fibronectin matrix assembly in control and transfected CHO cells. Confluent cell monolayers were cultured for 2 d with 200 nM human plasma fibronectin as described in MATERIALS AND METHODS. Cells were then fixed for 10 min in 3.7% (v/v) paraformaldehyde in PBS, and the fibronectin in the matrix was stained by a specific polyclonal antibody followed by a rhodamine-labeled anti-rabbit IgG. Note the dramatic reduction of fibronectin assembly in β1B-expressing cells. Bar, 80 μm.
