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. 1998 Apr;9(4):733–747. doi: 10.1091/mbc.9.4.733

Figure 1.

Figure 1

(A) Demembranated sperm heads of X. laevis as injected (DAPI stain for DNA). (B) Swollen sperm heads 5 min after injection into a Xenopus GV (DAPI stain). (C) Same field stained with mAb H14 against RNA Pol II; sperm heads are negative, whereas a coiled body (sphere) stains brightly (arrowhead). (D) Sperm head 3 h after injection into a GV (DAPI stain). (E) Same sperm head shows strong staining with mAb H14 against RNA Pol II. (F) Sperm heads 3 h after injection into a GV (DAPI stain). (G) Same field stained with mAb K121 against the trimethylguanosine (TMG) cap of snRNAs. Sperm heads are negative; B-snurposomes are stained (arrowheads). (H) Sperm heads 3 h after injection into a GV (DAPI stain). (I) Same field stained with mAb Y12 against the Sm epitope of snRNPs. Sperm heads are negative; B-snurposomes are stained (arrowheads).