Figure 5.

(A) Higher magnification of several sperm LBCs from the GV shown in Figure 4, stained with mAb H14 against RNA Pol II. The overall fuzzy appearance of the chromosomes is due to staining of the axes of the lateral loops. In addition, mAb H14 stains the terminal granules (arrowheads) found at the end of the long arm of 15 of the 18 Xenopus LBCs. (B) Single sperm LBC at still higher magnification, stained with mAb H14. The arrowhead points to the terminal granule, and arrows point to loop axes (DNA axes covered with Pol II).