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EGF-Induction of DNA Synthesis in Plcg1+/+ and Plcg1−/− cells. Confluent cells were incubated in DMEM plus 0.5% fetal bovine serum for 36 h before the addition of EGF (20 ng/ml) or no addition. At each indicated time, cells were incubated for 1 h with 3H-thymidine, and the radioactivity incorporated into trichloroacetic acid-insoluble material was determined as described in MATERIALS AND METHODS.
