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Induction of c-fos mRNA in Plcg1+/+ and Plcg1−/− cells. Confluent cells were incubated in media containing 0.5% fetal bovine serum for 72 h before the addition of EGF (50 ng/ml) for the indicated times. At each time point, total RNA was isolated and c-fos mRNA was detected by Northern blotting as described in MATERIALS AND METHODS. The blot was also probed for glyceraldehyde-3-phosphate dehydrogenase as a control for loading variation (lower panel).
