Table 4.
Allele | Survivors tested | Haploids | Diploids |
---|---|---|---|
1 | 5 | 0 | 5 |
6 | 10 | 0 | 10 |
412 | 5 | 0 | 5 |
737 | 24 | 9 | 15 |
1237 | 5 | 0 | 5 |
3796 | 5 | 0 | 5 |
Strains carrying the mps1-6 (AS181-2b), 1-412 (AS127-1d), 1-737 (AS132-8c), 1-1237 (AS126-5a), or 1-3796 (AS131-2d; Table 1) alleles were exposed to the nonpermissive temperature of 37°C for 4 h, and then plated for survivors at room temperature. Ploidy of survivors was determined by flow cytometry.