Figure 2.

Endoreduplication in Rcho-1 cells. (A) Differentiating Rcho-1 cells are postmitotic. The number of differentiated Rcho-1 cells was estimated at various times after the removal of the proliferating cells and plotted. Data represent means ± SE. (B) [³H]thymidine incorporation during Rcho-1 differentiation. The incorporation of [³H]thymidine into TCA-precipitable material was measured in Rcho-1 stem cells and in giant cells at d 0, 1, 2, 4, 6, and 8 after the removal of the proliferating cells. (C) DNA content of differentiating Rcho-1 cells measured by scanning cytometry. The DNA content of Rcho-1 cells was measured in the stem or giant cells on the indicated d of differentiation, and the average DNA content was expressed relative to the estimated haploid DNA content using serum-starved HeLa cells as a normal control for diploid DNA content.