Figure 7.

The production of GFP-Myr 1, GFP-Myr 1Δn295, and GFP-Myr 1 tail affects the distribution of transferrin receptor. Twenty-four hours after transfection with cDNA encoding GFP (A and B), GFP-Myr 1 (C and D), GFP-Myr 1Δn295 (E and F), and GFP-Myr 1-Tail (G and H), cells were fixed and permeabilized with saponin. Preparations were analyzed by epifluorescent microscopy, for both the expression of the GFP recombinant proteins (A, C, E, and G) and the distribution of endosomes bearing transferrin receptor (B, D, F, and H). Cells producing GFP proteins are indicated by arrows.