Figure 8.

In vitro competition of GST-BBMIΔ446 and GST-BBMI-Tail proteins with the MMIα associated with pools I–III. Membranes contained in the three pools were incubated in PBS at 0.5 mg/ml for 2 h at 4°C alone or with 2 μM GST-BBMIΔ446, GST-BBMI-Tail, or GST. After incubation with the fusion proteins, samples were centrifuged at 300,000 × g for 30 min. Proteins recovered from pellets and supernatants were separated by a 7% SDS-PAGE, transferred on nitrocellulose membrane, and probed with anti-MMIα antibodies (Tu 30). Samples loaded correspond to the treatment of 2.5 and 37.5 μg of total membrane proteins for the pellets and the supernatants, respectively.