Table 3.
Results for the split-ubiquitin system protein-protein interaction assays between GPA1 Nub fusion proteins and the candidate GPCR-Cub-PLV fusion proteins
| GPA1 Nub fusion orientations | |||||
| Locus tested (X-Cub-PLV) | Name | Nubwt-GPA1 | NubG-GPA1 | GPA1-Nubwt | GPA1-NubG |
| At1g57680.1 | Cand1 | ++ | - | ++ | - |
| At3g05010.1 | Cand2 | ++ | - | + | - |
| At3g59090.1 | Cand3 | ++ | - | ++ | - |
| At3g59090.3 | Cand5 | ++ | - | ++ | - |
| At5g18520.1 | Cand7 | ++ | - | ++ | - |
| At5g27210.1 | Cand8 | ++ | - | - | - |
| At4g21790.1 | TOM1 | ++ | - | ++ | - |
| At4g30850.1 | HHP2 | ++ | - | + | - |
Results were recorded after 3-5 days of yeast growth on minimal media containing 1 mM methionine to identify proteins that interact specifically with GPA1 as indicated by the extent of yeast growth. (+) indicates moderate growth while (++) indicate heavy yeast growth. Empty vector control plates did not show growth (data not shown). X-Cub-PLV, candidate GPCR-Cub-PLV fusions. Nubwt, wild type amino-terminal half of ubiquitin. NubG, reduced affinity mutant of Nubwt.