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. Author manuscript; available in PMC: 2008 Sep 5.
Published in final edited form as: Biochemistry. 2005 Dec 27;44(51):16844–16852. doi: 10.1021/bi051681j

Table 4.

Kinetic Parameters of PGDH with mutated Residues at the Serine Binding Site

Mutant Km
(mM)		 Vm
(mmol min−1 mg−1)		 kca
(sec−1)		 kcat / Km
(sec−1 M−1)		 I 0.5
(μM)		 nH
Native (4CA) 0.49 ± 0.03 4.61 ± 0.06 13.5 2.8 × 104 2.34 ± 0.4 1.88 ± 0.07
H344A 0.23 ± 0.03 3.35 ± 0.10 9.8 4.3 × 104 147.2 ± 4.4 1.70 ± 0.11
E345A 0.76 ± 0.06 4.07 ± 0.08 11.9 1.6 × 104 7.02 ± 0.11 1.80 ± 0.06
N346A 0.39 ± 0.05 5.08 ± 0.15 14.9 3.8 × 104 3138 ± 51 1.99 ± 0.07
R347A 0.45 ± 0.08 4.11 ± 0.08 12.1 2.7 × 104 5.5 ± 0.3 1.20 ± 0.10
P348A 0.58 ± 0.07 3.16 ± 0.08 9.3 1.6 × 104 10.11 ± 0.15 1.99 ± 0.07
G349V 0.69 ± 0.06 2.06 ± 0.04 6.0 0.9 × 104 238 ± 23 0.97 ± 0.11
N364A 0.29 ± 0.03 4.02 ± 0.09 11.8 4.1 × 104 15618 ± 313 2.12 ± 0.08