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. Author manuscript; available in PMC: 2008 Sep 5.
Published in final edited form as: Biochemistry. 2007 Aug 9;46(35):10170–10185. doi: 10.1021/bi700017z

Figure 1. ER stress-induced apoptosis in INS-1 cells.

Figure 1

INS-1 cells were treated with either vehicle (DMSO) or with thapsigargin (T, 1 μM) and incubated at 37 °C under an atmosphere of 95%CO2/5% O2 for up to 24 h. The cells were collected at 0, 12, and 24 h to determine the incidence of apoptosis. A. TUNEL-positive cells. Percentage of TUNEL-positive cells relative to total number of cells (DAPI-stained) were determined in a minimum of six fields on each slide from each experiment. Data represent mean ± SEM of values obtained from 5–7 separate experiments. (insert, iPLA2β-immunoreactive protein). (* Treated groups significantly different from corresponding 0 h control groups, p < 0.05. #OE treated group at 24 h significantly different from all other groups, p < 0.05.). B. DNA laddering. C. Mitochondrial membrane potential (MitoMP). Representative fluorescence spectra generated from analyses of 10,000 INS-1 cells from each experiment by flow cytometry are presented. M1 refers to the percentage of cells in which MitoMP is compromised. (Each assay was done 3–5 times.)