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Translocation of β-lactamase activity. Sec18–1 (A) and Sec63–1 (B) cells were incubated at 37°C, followed by addition of CHX and shift of the cells to 24°C. Intracellular (IN, filled circles) and secreted (EX, open circles) β-lactamase activity was determined. Parallel samples received DTT for the 37°C incubation. The intracellular activity (filled squares) was determined. No secreted activity was found in the presence of DTT.
