Skip to main content
. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2008 Apr 22;110(3-5):269–277. doi: 10.1016/j.jsbmb.2007.11.007

Figure 3.

Figure 3

Effects of 4 diastereoisomers of N-phenetyl-1α,25-(OH)2D3-26,23-lactam analogues on 1α,25-(OH)2D3-induced HL-60 cell differentiation as determined by NBT-reducing activity. (A) HL-60 cells were treated with 1α,25-(OH)2D3 alone or N-phenetyl-1α,25-(OH)2D3-26,23-lactam alone for 96 hrs, and NBT-reducing activity was examined. (B) HL-60 cells were treated with N-phenetyl-1α,25-(OH)2D3-26,23-lactam in the presence of 10−8M 1α,25-(OH)2D3 for 96 hrs, and NBT-reducing activity was examined. Rectangles and bars show mean ± S.D. of triplicate experiments, respectively. ap<0.001, compared with cells treated with media alone. *p<0.05, **p<0.01 and ***p<0.001, compared with cells treated with 10−8M 1α,25-(OH)2D3, respectively. Similar results were seen in two independent experiments.