Effect of (23S,25S)-DLAM-2P on reporter gene activity induced by 1α,25-(OH)2D3 in MVNP-transduced NIH3T3 cells. The reporter plasmid containing 25-OH-D3-24-hydroxylase promoter and β-galactosidase vector were transfected into MVNP-transduced NIH3T3 cells. 10−9M 1α,25-(OH)2D3 and various concentration of (23S,25S)-DLAM-2P were added for 24 hours after the transfection of MVNP-transduced NIH3T3 cells. Results are expressed as the mean ± SEM. ap<0.001, compared with cells treated with media alone. *p< 0.01 and **p<0.001 compared with cells treated with 10−9M 1α,25-(OH)2D3. Similar results were seen in three independent experiments.