Figure 7.

Expression of recombinant M-protein fragments in transiently transfected BHK-21/C13 cells. BHK-21/C13 cells transfected with constructs encoding Mp2 (A and B), Mp3 (C and D), or Mp2–Mp3 (E–H) were double stained with T7-tag antibody to localize the recombinant M-protein fragment (A, C, E, and G) and a titin antibody (B, D, F, and H). (A– D) Examples of transfected cells that contain large amounts of diffusely distributed recombinant protein (A and C) and titin, which appears in a normal staining pattern (arrowheads in B and D). The expressed Mp2–Mp3 construct either disrupts MLS, which then results in the appearance of numerous cytoplasmic aggregates containing both the recombinant protein and titin (arrowheads in E and F), or colocalizes with MLS (arrowheads in G and H). For details see RESULTS. Magnification, 1050×.